Abstract：Objective:To evaluate the effect of bul eyaconitine A(BLA) on adjuvant arthritis rats. Method:AA Wistar rats were induced by Freund’s complete adjuvant,Beginning from the onset of AA,AA Wistar rats were randomly assigned to bul eyaconitine A (at a dose of 50μg/kg (n=12),or 100μg/kg (n=12) ),or Aqua pro injection(at a dose of 100μL, n=12), once daily, respectively, for seven days.Increased percentage of body weight, change of circumference of ancle, and scores of arthritic flexion pain test were evaluated. The levels of TNF-α,INF-γ,PGE2 andβ-EP were measured with the use of ELISA. Result:A 100μg/kg of BLA can significantly reduce the circumference of arthritic ankle starting from the 2nd day (p<0.05), and the same result occurred at 50μg/kg of BLA from the 3rd day (p<0.05). BLA produced significant analgesia at a dose of 100μg/kg (p<0.05). The levels of TNF-α and PGE2 increased in 100μg/kg BLA treated rats (p<0.01). The levels of TNF-α in rats treated with 50μg/kg BLA decreased significantly as compared with Aqua pro injection group (p<0.01), and there was no difference between 50μg/kg group and 100μg/kg group.Conclusion:BLA can improve the inflammation of AA rats, which was correlated with its ability of reducing the TNF-αand PGE2 levels in serum. BLA can also reduce inflammatory pain by reducing the level of PGE2 and increasing the level ofβ-EP in serum.
2013, Vol. 11 
